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. Author manuscript; available in PMC: 2014 Oct 23.
Published in final edited form as: ACS Appl Mater Interfaces. 2013 Oct 8;5(20):10.1021/am401981g. doi: 10.1021/am401981g

Figure 5. Coating uniformity and cell viability of primary rat pancreatic islets following 3-layer encapsulation via PAMAM and alginate.

Figure 5

Single plane, confocal fluorescent images of rat islets cross-section 24 h post-encapsulation with 3-layer coatings (Images). Islet viability, via MTT metabolic assay, normalized to control (Graph). Groups: uncoated (Control); 3-layer coating using either PAMAM 15/0 (1), 15/20 (2), 15/30 (3), or 15/40 (4), interlayered with fluorescently-labeled hyperbranched Alg-N3; and NHS-PEG-N3, followed by 3-layer coating using PAMAM 15/40 interlayered with fluorescently-labeled hyperbranched Alg-N3. Error = standard deviation. Scale bar = 200 μm. * P < 0.05