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. Author manuscript; available in PMC: 2014 Oct 23.
Published in final edited form as: ACS Appl Mater Interfaces. 2013 Oct 8;5(20):10.1021/am401981g. doi: 10.1021/am401981g

Figure 6. Six-layer encapsulation of primary rat pancreatic islets via layer-by-layer assembly of alginate and PAMAM.

Figure 6

Evaluation of capsule formation via fluorescein labeled alginate and confocal z-stack projection (A–C) or single plane (a–c) imaging of rat pancreatic islets 24 hr after coating. Groups: (1) electrostatic assembly via three bilayers of PAMAM 15/0 and fluorescently-labeled hyperbranched Alg-N3; (2) primary layer of NHS-PEG-N3, followed by three bilayers of PAMAM 15/20 and fluorescentlylabeled hyperbranched Alg-N3; and (3)primary layer of NHS-PEG-N3, followed by three bilayers of PAMAM 15/40 and fluorescently-labeled hyperbranched Alg-N3. Scale bar = 50 μm