Figure 5.

Phosphate uptake in cystinotic and control ciPTEC. (A) Uptake of 0.2 mM ³²P-labeled phosphate during 5 min in cystinotic (red bars) and control (blue bars) ciPTEC was determined in triplicate with or without preincubation with 5 μM PSC883 or vehicle (0.1% DMSO) for 2 h. (B) Cystinotic cells were preincubated with 1 mM cysteamine (red bar) or vehicle (blue bar) (normal culture medium) for 1 h. Results are presented as % of ³²PO4 uptake (nmol/μg protein/5 min) *p < 0.05 vs. cystinosis.