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. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: Mol Cancer Ther. 2011 Mar 10;10(5):10.1158/1535-7163.MCT-10-0836. doi: 10.1158/1535-7163.MCT-10-0836

Figure 4. ZOL inhibits CCN1 expression in MDA-MB-231 cells.

Figure 4

Figure 4

A). MDA-MB-231 cells were transfected with the wild type CCN1-promoter (pGL3-CCN1-Luc) and after 24h cells were treated with increased concentrations of ZOL (10, 15, 25μM) for 24 hours and CCN1-promoter activity was measured. Luciferase activity was normalized by pRL-CMV control plasmid, which was co-transfected to normalize the plasmid transfection efficiency. The data represent means ± SE for 3 independent assays. *P< 0.05 from control cells. B). Subconfluent cells were treated with different concentrations of ZOL (10, 15 and 25μM) for 24h. Cells were lysed and total protein (30μg) was resolved by 10% polyacrylamide gel (Criterion XT Bis-Tris precast Gel) and analyzed by Western blotting with a rabbit polyclonal anti-CCN1antibody. Blots were reprobed with an antibody for β-actin as a control for protein loading.