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Determination of the exponential cycle of amplification. Semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) mixtures were taken for five alternating cycles starting at cycles 18, 21, 24, 27, and 30 for A: beta 2 microglobulin (B2M), B: superoxide dismutase 1 (SOD1), C: dual-specificity phosphatase 1 (DUSP1), and D: transforming growth factor, beta 1 (TGF-B1). Cycle 24 was selected as an exponential cycle.
