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. 2013 Dec 8;19:2501–2507.

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Copyright © 2013 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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The comparative mRNA expression of target genes in ten keratoconus (KCN) and pooled normal corneal tissues. This graph indicates the comparison of transcript band intensities for genes encoding superoxide dismutase 1 (SOD1), transforming growth factor, beta 1 (TGF-β1), and dual-specificity phosphatase 1 (DUSP1) between the KCN and non-KCN samples. The values were calculated relative to the beta 2 microglobulin (B2M) levels (as normalizer) and reported as mean percentages. Note that the KCN corneas showed a three- and fivefold increase in TGF-B1 and DUSP1 when compared to the pooled normal corneas. The RNA levels for SOD1 were similar for the KCN and normal corneas (p<0.05). Normal corneal samples were pooled to reduce tissue preparation errors. The error bars represent the standard deviation.