Table 1. RT–PCR Primers Used for B2M, TGFB1, DUSP1 and SOD1.
Target gene | Primer sequences | PCR product size |
---|---|---|
B2M
(NM_004048.2) |
F: 5′-TATCCAGCGTACTCCAAAGA-3′
R: 5′-GACAAGTCTGAATGCTCCAC-3′ |
191 |
TGFB1
(NM_000358) |
F: 5′-ATCACCAACAACATCCAGCA-3′
R: 5′-CCGTTACCTTCAAGCATCGT-3′ |
165 |
DUSP1
(NM_004417) |
F: 5′-GAGGGTCACTACCAGTACAAGAGC-3′
R: 5′-GCCTGGCAGTGGACAAACA-3′ |
137 |
SOD1 (NM_000454) | F: 5′-CAGTGCAGGGCATCATCAAT-3′ R: 5′-CATTGCCCAAGTCTCCAACA-3′ | 222 |
All primers are from 5′ to 3′. β2M, beta-2-microglobulin; SOD1, superoxide dismutase1; DUSP1, Dual-specificity phosphatases; TGF-β1, Transforming growth factor beta1