Figure 5. Apyrase treatment reduces [Ca²⁺]_r in FDB adult fibers.

Adult fibers were isolated from wt and mdx mice and incubated in Krebs Ringer solution with or without apyrase (2 U/mL) for 10 min at room temperature. [Ca²⁺]_r was determined by double-barreled Ca²⁺ selective microelectrodes. Data are expressed as mean ± S.E.M. from n fibers (indicated in the figure) from three different cultures. n.s, no significant difference, ***P<0.001, ANOVA-Tukey’s.