Figure 7. Fibrinogen binding of mutant 321/358 after priming.

HEK293 cells expressing normal αIIbβ3 or mutant 321/358 were incubated with buffer (Control), eptifibatide (1 µM), RGDS (100 µM), RUC-1 (100 µM), RUC-2, (1 µM), or EDTA (10 mM) for 20 min at RT and then fixed and washed 4 times. Binding of Alexa488-conjugated fibrinogen (200 µg/ml) was then assessed by flow cytometry and expressed as NNFI, in which the net geometric mean fluorescence intensity after subtracting the background is divided by the relative surface receptor expression as judged by the binding of mAb 10E5. Data expressed as mean ± SD after subtracting the EDTA value as background (n = 3).