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. 2013 Oct 31;27(12):2041–2054. doi: 10.1210/me.2013-1191

Figure 4.

Figure 4.

Validation of microarray results with qPCR, ELISA, and Western blotting after 3 days of treatment with deciduogenic hormones. A, Fold change in mRNA abundance in COUP-TFII siRNA relative to nontargeting siRNA in HESCs. B and C, Secreted protein levels of IL-6 and IL-8 in HESC culture media. Forty-eight hours after transfection (defined as day 0) with nontargeting siRNA (siNT) or COUP-TFII siRNA (siCII), HESCs were treated with deciduogenic hormones for 3 days. The culture media were collected on day 3, and the levels of IL-6 and IL-8 were measured by ELISA. D, Protein levels of COUP-TFII and PGR isoforms on day 0 and day 3 of treatment with deciduogenic hormones between COUP-TFII siRNA (siCII) and nontargeting siRNA (siNT) normalized to glyceraldehyde-3-phospate dehydrogenase (GAPDH). *, P < .05; **, P < .01.