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. 2013 Dec 9;8(12):e81220. doi: 10.1371/journal.pone.0081220

Figure 4. The effect of NOR on the VEGF-induced intracytoplasmic cAMP level and PKA activation in HUVECs.

Figure 4

(A). Effect of NOR on VEGF-induced intracytoplasmic cAMP level. Cells were treated with NOR and VEGF (10 ng/ml) for 24 h. (B). Effect of NOR on VEGF-induced PKA catalytic activity. Cells were treated with NOR and VEGF (10 ng/ml) for 24 h. (C). Effect of NOR on VEGF-induced PKA phosphorylation. Cells were pretreated with NOR for 24 h, stimulated by VEGF for 20 min, and then harvested for western blot analysis. (D). Quantification of PKA phosphorylation. The data are expressed as mean ± SD of three independent experiments. # P<0.05, ## P<0.01 vs. normal group; *P<0.05, **P<0.01 vs. VEGF group. NOR, norisoboldine; Cur, curcumin.