Figure 7. The effect of NOR on the VEGF-induced activation of the NF-κB pathway.

(A). Effect of NOR on VEGF-induced expressions of IκBα and p-IκBα in HUVECs. (B). Quantification of IκBα and p-IκBα expressions. (C). Effect of NOR on the disruption of p65/IκB complex in VEGF-activated HUVECs. Cells were treated with NOR or Cur in the presence of VEGF for 24 h. Then cell lysates were immunoprecipitated with IκBα antibody and immunoblotted with p65 antibody. The data were expressed as mean ± SD of three independent experiments. ^## P<0.01 vs. normal group; *P<0.05, **P<0.01 vs. VEGF group. NOR, norisoboldine; Cur, curcumin.