Figure 3. Expression analysis of MYBL2 protein after treatment with paclitaxel (Taxol, T) and doxorubicin (Adriamycin, A) in several cell lines by Western blotting (non-tumorigenic cell line MCF-10A and breast cancer cell lines MCF-7, BT-20 and SKBR3).

Single treatment with T or A for 48(T (48 h); A (48 h)), combined treatment for 48 h (T + A (48 h)) or successive treatment for each for 24 h (T (24 h), A (24 h) was applied. Quantification of western blotting results was carried out with individual passaged cells for at least three times. Representative western blots were displayed on top of the graphs. Proliferative alterations were detected against Proliferating Cell Nuclear Antigen (PCNA). Loading controls were labeling of the house keeping protein β-actin and stain-free imaging of the SDS-PAGEs prior blotting procedure. Mean ± SD values (n = 3). * : p<0.05; ** : p<0.01; * * * : p<0.001 as compared to control treatment (unpaired t test).