Figure 5. Localization of SIV-specific CD8⁺ T cells in lymph nodes and spleen.

The representative images shown in are A) from an axillary lymph node from an animal (119.96) 5 weeks post-SIV-Δnef vaccination. Mamu-A1/Tat tetramer⁺ cells are red to pink, CD8⁺ T cells are green, and CD3⁺ T cells are blue. The left panel shows a montage of several fields containing projected z-scans collected with a confocal microscope 20× objective. Enlargements to the right show individual fields of projected z-scans collected with a 60× objective. The representative images shown in B) are from a spleen section from an animal (119.96) at 5 weeks post-SIVΔnef vaccination. Mamu-A1/Gag tetramer⁺ cells are red to pink colored, CD8⁺ T cells are blue, and CD20⁺ B cells are green. Tetramer⁺CD8⁺ cells localized with other CD8 T cells and were most abundant in the red pulp (RP) and marginal zones (MZ), with relatively fewer located in T cell zones (T) and in B cell follicles (B). The left panel shows a montage of several confocal microscope fields of projected Z-scans stitched together, collected with a 10× objective. Enlargements show individual confocal z-scans of single 200× fields.