Skip to main content
. 2013 Dec 9;8(12):e81719. doi: 10.1371/journal.pone.0081719

Figure 1. Kinetics of Peyer's Patch Infection with B. melitensis 16 M.

Figure 1

Jejunal-ileal loops were intraluminally inoculated in 3 ml containing 1×109 CFU of B. melitensis 16 M/ml. Tissue (Peyer's patch) samples of 0.1 g of mucosal tissue were extracted at 0.25, 0.5, 1, 2, 4, 8 and 12 h from every infected loop, intensively washed 3 times in PBS, macerated and diluted in 1 ml of distilled water. To evaluate the kinetics of the infection, macerated samples were serially diluted and cultured on Farrell's medium. Numbers of CFU recovered from bovine Peyer's patches are the average of 4 calves. Bars represent standard deviation.