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. 2013 Dec 9;8(12):e82509. doi: 10.1371/journal.pone.0082509

Figure 6. Inhibitory effects of vinpocetine on Akt/IKKα/β/IκB phosphorylation are independent of a PDE1B-mediated cGMP increase mechanism.

Figure 6

A. Total RNA is isolated from peripheral blood mononuclear cells (PBMCs) or macrophages, and the expression of PDE1B is determined by PCR. B. Positive effect of vinpocetine on intracellular cGMP contents. PMA-induced THP-1 macrophages are pretreated with various doses of vinpocetine or vehicle for 15 and 30 min. C. The expression levels of PDE1B after transfection with scramble and PDE1B siRNA are determined by PCR. GAPDH indicates house-keeping gene glyceraldehyde-3-phosphate dehydrogenase. D. Knockdown of PDE1B leads to an increase in intracellular cGMP contents. E and F. Knockdown of PDE1B does not alter the phosphorylation levels of Akt, IKKα/β and IκB.

Each experiment is repeated four times. Bars indicate mean ± SEM. 0 indicates vehicle DMSO. * P < 0.005 vs. control. # P < 0.005 vs. ox-LDL + vehicle.