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. 2013 Dec 9;8(12):e82819. doi: 10.1371/journal.pone.0082819

Figure 3. Cellular death assessment in organotypic culture of fresh pre-pubertal mouse testicular tissue.

Figure 3

Cellular death was assessed in organotypic culture at days 0, 7, 9 and 11 on 30 cross-sectioned seminiferous tubules after Tra98 immunolabelling under the different culture conditions tested.

(A) Percentage of total pyknotic seminiferous tubules.

(B) Number of pyknotic nuclei per seminiferous tubules.

The results are represented as the mean ± SEM with n=6.

Footnotes:

T: basal culture medium without retinoid; RARE: 3.3.10-7M RA and 3.3.10-7M RE; RARE6: 3.3.10-7M RA and 10-6M RE; RARE5: 3.3.10-7M RA and 10-5M RE; RERA6: 3.3.10-7M RE and 10-6M RA; RERA5: 3.3.10-7M RE and 10-5M RA; RA6: 10-6M RA; RE6: 10-6M RE; RE5: 10-5M RE; RE4: 10-4M RE; In vivo: In vivo control.

RA: Retinoic acid; RE: Retinol; D0: Day 0; D7: Day 7; D9: Day 9; D11: Day 11