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. 2013 Dec 9;8(12):e82819. doi: 10.1371/journal.pone.0082819

Figure 5. PCNA (Proliferating Cell Nuclear Antigen) expression in organotypic culture of fresh pre-pubertal mouse testicular tissue.

Figure 5

PCNA expression was assessed in organotypic culture at days 0, 7, 9 and 11 on at least 500 intra-tubular cells under the different culture conditions tested. The results are represented as the mean ± SEM with n=6.

(A) Percentage of total PCNA-positive cells.

(B) Percentage of PCNA-positive Sertoli cells.

(C) Percentage of PCNA-positive germ cells.

FootnotesT: basal culture medium without retinoid; RARE: 3.3.10-7M RA and 3.3.10-7M RE; RARE6: 3.3.10-7M RA and 10-6M RE; RARE5: 3.3.10-7M RA and 10-5M RE; RERA6: 3.3.10-7M RE and 10-6M RA; RERA5: 3.3.10-7M RE and 10-5M RA; RA6: 10-6M RA; RE6: 10-6M RE; RE5: 10-5M RE; RE4: 10-4M RE; In vivo: In vivo control.

RA: Retinoic acid; RE: Retinol; D0: Day 0; D7: Day 7; D9: Day 9; D11: Day 11