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. 2013 Dec 9;8(12):e82819. doi: 10.1371/journal.pone.0082819

Figure 8. Meiotic process in organotypic culture of fresh pre-pubertal mouse testicular tissue at days 0, 7, 9 and 11.

Figure 8

Meiotic germ cells assessment performed after Tra98 immunodetection on 30 cross-sectioned seminiferous for each tested concentration of retinoids. The results are represented as the mean ± SEM with n=6.

(A) Percentage of seminiferous tubules in meiotic prophase (i.e containing leptotene, zygotene or pachytene primary spermatocytes).

(B) Percentage of primary spermatocytes (i.e. leptotene, zygotene or pachytene) in meiotic seminiferous tubules.

FootnotesT: basal culture medium without retinoid; RARE: 3.3.10-7M RA and 3.3.10-7M RE; RARE6: 3.3.10-7M RA and 10-6M RE; RARE5: 3.3.10-7M RA and 10-5M RE; RERA6: 3.3.10-7M RE and 10-6M RA; RERA5: 3.3.10-7M RE and 10-5M RA; RA6: 10-6M RA; RE6: 10-6M RE; RE5: 10-5M RE; RE4: 10-4M RE; In vivo: In vivo control.

RA: Retinoic acid; RE: Retinol; D0: Day 0; D7: Day 7; D9: Day 9; D11: Day 11