Table 1. Culture media for fresh immature mouse testicular tissue offering the best results for each studied parameter (seminiferous tubule area, intra-tubular cell proliferation, necrosis, cellular differentiation) after 11 days of organotypic culture.
| Studied parameter | Most favourable culture condition |
|---|---|
| Seminiferous tubule area | RE6, RERA6, RARE5, RARE6 |
| Intra-tubular cell proliferation | RARE6, RE6, RARE, RERA6 |
| Necrosis | RARE, RARE6 |
| Cellular differentiation | RARE, RE6, RERA6, RARE5 |
| Ratio between Sertoli cells and spermatogonia | RE6, RARE5 |
Footnotes:
RARE: 3.3.10-7M RA and 3.3.10-7M RE; RARE6: 3.3.10-7M RA and 10-6M RE; RARE5: 3.3.10-7M RA and 10-5M RE; RERA6: 3.3.10-7M RE and 10-6M RA; RE6: 10-6M RE.
RA: Retinoic acid; RE: Retinol