Figure 4. Interation with lipid.

II. (A) Direct interaction of amylolysin with lipid II. Both compounds were incubated in DMSO/1-octanol mixture (60/40 v/v) for 1 hour at room temperature before being subjected to TCL using a butanol/acetic acid/pyridine/water mixture (15/3/12/10, v/v/v/v) as mobile phase. (B) Inhibition of the transglycosylation reaction. Amylolysin, lipid II and glycosyltransferase were incubated for one hour before being subjected to TLC using a mixture of methanol/chloroform/ammoniac/water (88/48/10/1, v/v/v/v) as mobile phase. [¹⁴C] lipid II was detected with a Molecular Imager FX system. Direction of solvent migration during TLC is indicated by the arrow.