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. 2013 Dec 9;8(12):e83191. doi: 10.1371/journal.pone.0083191

Figure 3. Partial restoration of L. monocytogenes L.p.FlaA virulence after MMC depletion.

Figure 3

8-10 week old CD11c-YFP transgenic x LysM+/eGFP mice were treated with 250µg RB6-8C5 mAb and then infected i.v. with 2.5x104 wt or 1x105 L. monocytogenes L.p.FlaA both expressing RFP for up to 48 hours. After infection spleens were excised, sectioned, and image using multi-photon microscopy. (A) Multi-photon images of CD11c-YFP transgenic x LysM+/eGFP mice at 48 hours after infection. L. monocytogenes-RFP, MZ-705 quantum dots-yellow. (B) Relative RFP intensity inside individual WP nodule (A.U.). Data representative of 3 independent experiments. (C) Bacterial CFU from liver and spleen at the indicated time points after RB6-8C5 antibody treatment. The median of at least three replicates from 3 independent experiments. *P < 0.05 by Mann-Whitney test.