Figure 5. CD8α⁺ DCs harbor significantly less after L. monocytogenes inflammasome activation.

(A) 8-10 week old C57Bl/6 mice were infected intravenously with PBS, 2.5x10⁴ wt or 1x10⁵ L. monocytogenes L.p.FlaA for 12 and 18 hours. After infection, spleens were harvested, dissociated, and isolated DCs were stained with CD8α⁺, CD11b⁺, CD11c+, CD86⁺ antibodies and analyzed using FACS. Bar graph of percentage of CD11c^hiCD8α^hi DCs. The average ± SD of three replicates from 3 independent experiments. (B) Histogram of CD86 expression in CD11c^hiCD8α^hi DCs. (C) 8-10 week old C57Bl/6 mice were infected intravenously with PBS, 2.5x10⁴ wt or 1x10⁵ L. monocytogenes L.p.FlaA for 24 hours. After infection, spleens were harvested, dissociated, and CD8α⁺ DCs were purified using bead purification. Total isolated spleens were plated on BHI with bacterial counts taken after 24 hours. The average ± SD of four replicates from 4 independent experiments. (D) C57Bl/6 mice were treated for 5 hours with 250µg RB6-8C5 mAb and then infected intravenously with PBS, 2.5x10⁴ wt or 1x10⁵ L. monocytogenes L.p.FlaA for 24 hours. After infection, spleens were harvested, dissociated, and CD8α⁺ DCs were purified using bead purification. Total isolated spleens were plated on BHI with bacterial counts taken after 24 hours. The average ± SD of four replicates from 4 independent experiments.