Figure 3. Biallelic expression of KCNQ1OT1 in LOS fetuses. Shown is Sanger sequencing data of KCNQ1OT1 RT-PCR product in tissues analyzed in AI-B799 (control), ART-J835LOS and ART-J489ALOS fetuses. The columns show the chromatograph for each tissue of each fetus. Values below the chromatograph are the percentage of KCNQ1OT1 expressed from the maternal allele. Arrows show the double peaks of SNP1 site (refer to Fig. 2) in ART-J835LOS and ART-J489ALOS fetuses. For clarity of depiction, SNP2 site is shown here (Fig. S1). LOS, large offspring syndrome; RT-PCR, reverse transcription-polymerase chain reaction; SNP, single nucleotide polymorphism.