Figure 4. Bisulfite sequencing of the GPR1AS promoter region in human placenta and umbilical blood samples. Each row represents the results of an independent sequencing reaction. Open and closed circles denote unmethylated and methylated CpGs. Calculated methylation levels are shown below each graph. Heterozygous SNP rs1683074 was used to distinguish between maternal and paternal alleles in human samples. One of case (Family ID: 055) was also investigated by allelic expression analysis in Figure 3.