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. 2013 Jun;2(5):215–224. doi: 10.1089/wound.2012.0406

Figure 1.

Figure 1.

Quantitative analysis of the stratifin-stimulated matrix metalloproteinase (MMP)–1 mRNA in fibroblasts in the presence and absence of insulin-like growth factor (IGF)–1 and transforming growth factor (TGF)–β1. Three different strains of primary dermal fibroblasts isolated from 3 different individuals were either left untreated or treated for 24 h with stratifin—alone, or in the presence of IGF-1 (100 ng/mL), TGF-β1 (100 pg/mL), or both. Cells were then harvested, total RNA was extracted, and the expression of MMP-1 mRNA was evaluated by Northern blot analysis. (A) The representative pattern of MMP-1 mRNA, as well as that of 18S ribosomal RNA, which was used as loading control. (B) The expression of MMP-1 mRNA was then quantified by densitometry, and the mean±SD obtained from three separate experiments was then expressed as percentage of their corresponding controls. Significant difference was found in comparing *between untreated and stratifin-treated samples (data on lane 1 vs. 2) and **between the stratifin-treated and stratifin+TGF-β1+IGF-1–treated cells (data on lane 2 vs. 5).