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. 2013 Jun;2(5):215–224. doi: 10.1089/wound.2012.0406

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Copyright 2013, Mary Ann Liebert, Inc.

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Figure 3. — Differentiation of keratinocytes is associated with an increase in the mRNA expression of stratifin while decreasing the expression TGF-β1 mRNA. Keratinocytes were cultured in test medium consisting of 49% keratinocytes serum free medium, 49% Dulbecco's modified Eagle's medium, and 2% fetal bovine serum for different durations. Keratinocytes were then harvested and evaluated for the mRNA expression for (A) involucrin as an index for keratinocyte differentiation, (B) stratifin, and (C) TGF-β1. Each blot was then evaluated for the expression of 18S RNA, which was used as a loading control.