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. 2011 Jul 23;51(1):16–24. doi: 10.1007/s13197-011-0455-4

Table 1.

Conditions for hydrolysis using four microbial proteases

Alcalase Neutrase Protamex Flavourzyme
pH 7.0–8.0 6.3–6.5 7.2–8.0 5.5–7.5
Temperature 56–60 47–50 50–52 50–55
[E]/[S] ratio 30a 30a 100a 125b

aExpressed as AU kg −1 protein

bExpressed as LAPU kg −1 protein