Table 3.
Box–behnken design matrix and the responses of the dependent variable degree of hydrolysis (%DH) for shrimp waste hydrolysis by Alcalase
| Run | T | pH | E/S | t | DH % |
|---|---|---|---|---|---|
| 1 | 1 | 1 | 0 | 0 | 29.9 |
| 2 | 0 | 0 | −1 | −1 | 4.2 |
| 3 | 0 | 0 | −1 | 1 | 17.3 |
| 4 | 0 | 0 | 1 | −1 | 15.4 |
| 5 | 0 | 0 | 1 | 1 | 22.5 |
| 6 | −1 | 0 | 0 | −1 | 2.3 |
| 7 | −1 | 0 | 0 | 1 | 11.4 |
| 8 | −1 | −1 | 0 | 0 | 25.0 |
| 9 | −1 | 1 | 0 | 0 | 23.1 |
| 10 | 1 | −1 | 0 | 0 | 10.1 |
| 11 | 1 | 0 | 0 | −1 | 32.9 |
| 12 | 1 | 0 | 0 | 1 | 7.6 |
| 13 | 0 | −1 | −1 | 0 | 19.1 |
| 14 | 0 | −1 | 1 | 0 | 14.5 |
| 15 | 0 | 1 | −1 | 0 | 24.8 |
| 16 | 0 | 1 | 1 | 0 | 19.5 |
| 17 | 1 | 0 | −1 | 0 | 16.2 |
| 18 | 1 | 0 | 1 | 0 | 28.0 |
| 19 | 0 | −1 | 0 | −1 | 7.8 |
| 20 | 0 | −1 | 0 | 1 | 15.4 |
| 21 | 0 | 1 | 0 | −1 | 13.0 |
| 22 | 0 | 1 | 0 | 1 | 27.6 |
| 23 | 0 | 0 | 0 | 0 | 20.6 |
| 24 | 0 | 0 | 0 | 0 | 20.2 |
| 25 | 0 | 0 | 0 | 0 | 20.4 |
| 26 | −1 | 0 | −1 | 0 | 13.5 |
| 27 | −1 | 0 | 1 | 0 | 24.0 |
| 28 | −1 | 1 | 1 | 0 | 23.1 |
| 29 | −1 | −1 | 0 | −1 | 19.0 |
| 30 | 1 | 0 | 0 | 0 | 28.0 |
*Average of duplicate determinations from different experiments.T, temperature; E/S, enzyme/substrate ratio (%v/w of shrimp waste); t, time