Table 4.
Analysis of variance for the response of degree of hydrolysis
| Source | Degree of freedom | Sum of squares | Mean square | F-value | P value |
|---|---|---|---|---|---|
| Model | 14 | 1205.20 | 86.09 | 2.51 | <0.0001 |
| A (Reaction temperature) | 1 | 152.20 | 152.20 | 4.43* | 0.17456 |
| B (pH) | 1 | 105.16 | 105.16 | 3.06* | <0.0001 |
| C (Reaction time) | 1 | 8.45 | 8.45 | 0.25* | 0.08546 |
| D (Enzyme/substrate ratio) | 1 | 2.06 | 2.06 | 0.60* | <0.0001 |
| AB | 1 | 0.022 | 0.022 | 6.537** | 0.00856 |
| AC | 1 | 111.89 | 111.89 | 3.26** | 0.00254 |
| AD | 1 | 10.95 | 10.95 | 0.32** | 0.17652 |
| BC | 1 | 52.99 | 52.99 | 1.54** | 0.84634 |
| BD | 1 | 3.77 | 3.77 | 0.11** | 0.45233 |
| DC | 1 | 112.86 | 112.86 | 3.29** | 0.94572 |
| A2 | 1 | 15.63 | 15.63 | 0.46* | 0.23659 |
| B2 | 1 | 30.94 | 30.94 | 0.90* | <0.0001 |
| C2 | 1 | 24.04 | 24.04 | 0.70* | 0.23451 |
| D2 | 1 | 59.44 | 59.44 | 1.73* | <0.0001 |
| Residual | 15 | 514.93 | 34.33 | 34.33 | – |
| Pure error | 4 | 55.63 | 13.91 | 13.91 | – |
| Lack of fit | 11 | 459.30 | 41.75 | 41.75 | 0.14999 |
| Total | 29 | 1720.14 |
% variability explained R2 = 0.9554, R2 adj = 0.9213
* Significant at α = 0.01
** Significant at α = 0.05