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. 2013 Dec 9;203(5):801–814. doi: 10.1083/jcb.201308001

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© 2013 Wang et al.

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Figure 1. — ER network formation in crude interphase X. laevis egg extracts. Demembranated sperm was added to a crude interphase X. laevis egg extract containing Alexa fluor 488–labeled tubulin and the hydrophobic dye DiIC₁₈. The formation of MT asters (A) and ER network (B) was followed over time by confocal fluorescence microscopy (for full time course, see Videos 1 and 2). Sperm was added at time zero. Bars, 30 µm. (C) As in A and B, but after 60 min, a time point at which the NE has formed. Bars, 20 µm.