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. 2013 Dec 9;203(5):801–814. doi: 10.1083/jcb.201308001

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© 2013 Wang et al.

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Figure 3. — The leading ends of ER tubules track with the plus ends of MTs. (A) Demembranated sperm was added for 30 min to a crude interphase X. laevis egg extract containing the hydrophobic dye DiIC₁₈ and a GFP fusion of the plus end tracking protein EB-1. The movements of ER tubules and of the plus ends of MTs were followed over time by confocal fluorescence microscopy (for full time course, see Video 4). Yellow arrows indicate the leading ends of ER tubules (red) that track with the plus ends of growing microtubules (green). The blue arrow indicates the site of attachment between one of the extending ER tubules and another tubule to form a three-way junction. Bars, 2 µm. (B) Quantification of experiments such as shown in A. The percentage of outward moving ER tubules that tracked with EB-1 is shown. Error bars indicate the range within three independent experiments, with 50 tubule-extension events analyzed in each experiment.