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. 2013 Dec 9;203(5):801–814. doi: 10.1083/jcb.201308001

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© 2013 Wang et al.

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Figure 7. — ATL mediates the fusion of membranes into an ER network. (A) A crude interphase extract was preincubated for 5 min with 2 µM of an inactive mutant of the cytosolic fragment of ATL (ATL(R232Q)). Then, membranes were added that were separately prestained with DiIC₁₈ (red) and DiOC₁₈ (green). The sample was imaged after 30-min incubation by confocal microscopy with a short exposure time (40 ms). (B) As in A, but with wild-type cytosolic fragment of ATL (cytATL). Insets in A and B show a magnified view of the boxed areas. (C) As in A and B, but with membrane-depleted interphase cytosol and a mixture of light membranes that were prestained with either DiIC₁₈ or DiOC₁₈. Insets show a magnified view of the boxed areas. Bars: (A and B) 20 µm; (A and B, insets) 10 µm; (C) 10 µm; (C, insets) 5 µm.