Figure 1.

CEP152-KO and STIL-KO DT40 cells lack intact centrioles. (A–G) TEM of WT, CEP152-KO, and STIL-KO cells. Arrowheads indicate centriolar satellites. Bars, 100 nm. (A) A centriole pair is illustrated in WT. (B and C) CEP152-KO cells contain electron-dense structures reminiscent of partial centriole walls. Insets in A and C show a cross section of triplet MTs at high magnification. (D–F) STIL-KO cells lack centriolar structures and contain only aMTOCs. (F) Mitotic spindle pole in STIL-KO. (G) Table depicts quantification of the major phenotypes observed by TEM. Asterisks indicate electron-dense structures that can potentially correspond to centrioles.