Figure 3.

Cells lacking intact centrioles proliferate slowly but are proficient in DNA repair. (A) CEP152-KO and STIL-KO cells display growth impairment. (B) KO cells show increased apoptosis compared with WT. n = 4. Error bars indicate SEMs, analysis of variance, Tukey’s test. (C) Quantification of cell cycle profiles by FACS analysis with propidium Iodide (PI) and anti-BrdU antibodies, with >15,000 events per genotype. Percentages of cells per each cell cycle stage are indicated. (D) Exponential one phase decay graph illustrates clonogenic survival of WT, CEP152-KO, and STIL-KO cells after genotoxic treatments. The percentage of colony formation was normalized against untreated controls of the same genotype. Cells were treated with indicated doses of cisplatin for 1.5 h or irradiation (IR). Error bars indicate SDs.