Figure 5.

CEP152-KO and STIL-KO cells exhibit chromosome instability and aneuploidy. (A) Still frames from time-lapse experiments show H2B-GFP–expressing WT, CEP152-KO, and STIL-KO cells (Videos 4, 5, and 6). Lagging chromatids (red arrowheads) or larger chromatin masses (yellow arrowheads) are evident during anaphase in KO cells. Pictures with blue asterisks are shown with increased gain setting in the framed area. Bars, 2 µm. (B) Table summarizes chromosome missegregation phenotypes from time-lapse experiments. Overlays of H2B-GFP and bright-field images were analyzed. (C) Lagging chromatids in anaphase cells show merotelic attachments. Cells were stained with antibodies against CENP-O and α-tubulin. DNA is shown in blue. Framed areas are shown at higher magnification. Bars, 5 µm. (D) Chromosome (chr) spreads of WT and CEP152-KO cells. WT cells show normal ploidy. CEP152-KO image on the left illustrates CA (trisomy of chromosome 3 but normal copy number of chromosomes 1, 2, 4, and Z) and, on the right, CIN (trisomy of chromosome 4 but normal copy number of chromosomes 1, 2, 3, and Z). Pie charts on the bottom depict the percentage of cells with indicated ploidies. Numbers of chromosomes 1–4 and Z were scored. Bar, 8 µm.