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. 2013 Dec 9;203(5):785–799. doi: 10.1083/jcb.201305109

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© 2013 Franz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Cellular CP110 levels influence centriole length. (A–M) Panels show representative EM images of centrioles in larval wing disc cells of various genotypes (as indicated on each panel); n (total centrioles; total wing discs): 134;9 (A), 127;9 (B), 45;4 (C), 53;3 (D), 61;5 (E), 75;5 (F), 109;9 (G), 48;3 (H), 98;8 (I), 87;7 (J), 75;5 (K), 42;4 (L), and 93;7 (M). Arrows highlight MT extensions. (N and O) Bar charts show the quantification of centriole length in these genotypes. Note that the overexpression of CP110S-GFP dramatically shortens centrioles in a WT background, but has a less marked effect in the CP110Δ background (only bordering on the statistically significant). We suspect that this might be because MT extensions are still present in CP110Δ cells overexpressing CP110S-GFP, potentially contributing to the slight lengthening of the centriole. Bar, 100 nm.