Fig. 2.

Detection of Plasmodium spp. and P. ovale wallikeri DNA by nested PCR assays. Molecular size markers in base pairs (bp) are shown in the end lanes (M). A: rFAL 1 and rFAL 2, P. falciparum-specific primers (205 bp); rVIV 1 and rVIV 2, P. vivax-specific primers (144 bp); rMAL 1 and rMAL 2, P. malariae-specific primer (112 bp); rOVA 1 and rOVA 2, P. ovale curtisi-specific primers (800 bp); rOVA 1v and rOVA 2v, P. ovale wallikeri-specific primers (782 bp); rPLU 3 and rPLU 4, Plasmodium-specific primers (240 bp). B: HNS: Hainan sample; SHS1 and SHS2: the first and second delivery samples from Shanghai Center for Disease Control and Prevention, respectively.