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. 2013 Dec 10;4:362. doi: 10.3389/fphys.2013.00362

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Copyright © 2013 Ip, Hiong, Wong, Ching, Chen, Soh, Chng, Ong, Wilson and Chew.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of acclimation to brackish water on Na⁺:K⁺:2Cl⁻cotransporter 1 (nkcc1) mRNA expression in the gills of Himantura signifer. Absolute quantification (copies of transcript per ng of cDNA) of mRNA expression of nkcc1 in gills of H. signifer kept in fresh water (FW; control) or exposed to brackish water (salinity 20) for 1 or 6 d. Results represent means + s.e.m. (N = 5). Means not sharing the same letter are significantly different (P < 0.05).