Table 1.

Primer sequences for PCR, RACE-PCR and quantitative real-time PCR (qPCR).

Gene	Primer type		(5′ → 3′)
nkcc1	PCR	Forward	GCWGCCACWGGYATT
		Reverse	GTSCCYTTSCCCTGHTTCTTCTG
	RACE-PCR	5′RACE	AGTTGTGCAGTTAGTCACTGTTCCAG
		3′RACE	ACGCAAGAACACAAGGATGAAGAAGATG
	qPCR	Forward	GTCGTCACCACCATCACAG
		Reverse	TCCAATAGCTCCTCCAAATTCAG
nkaα	PCR	Forward	CACTTCATCCACATCATCAC
		Reverse	ATGGCAGGGAACCATGTC
nkaα1	RACE-PCR	5′RACE	CCTGGAAGACTGCCCGGTTGCACA
		3′RACE	ACGCCATCATACTGAGATAGTCTTTGCT
	qPCR	Forward	AGTCTTCCAGGCAGGGCA
		Reverse	GCTCAACTGATCCACAACACAA
nkaα2	RACE-PCR	5′RACE	TGGGTGAAGTCTTATCAAAGGCCGAA
		3′RACE	AAGAAGAGCAGCTAGACCAGATCCTG
	qPCR	Forward	GCCTTTGATAAGACTTCACCCAC
		Reverse	GCTGTCTCACGCTTGGAAAT
nkaα3	RACE-PCR	5′RACE	TTTGTCGAAGGAAGCACCGGACTGG
		3′RACE	GAGCAGATTGACGACATCCTCCGG
	qPCR	Forward	GCGAGACAAGAACAAGAAGATT
		Reverse	GCCTCCTTCATCTCCTCATC

Primers used for PCR, RACE and qPCR of Na⁺:K⁺:2Cl⁻ cotransporter 1 (nkcc1) and Na⁺/K⁺-ATPase (nka) α-subunit isoforms from gills of Himantura signifer.