Figure 5. Live imaging reveals that CMT2B Rab7 proteins exhibit defective endosomal recruitment but do not affect endosomal maturation.
(A–F) Snapshots from live imaging datasets for three genotypes with a time lapse of 30 s (A and D) Venus-Rab7-WT and spin-RFP; (B and E) Venus-Rab7-Q67L and spin-RFP; (C and F) Venus-Rab7-N161T and spin-RFP. Arrows mark individual Rab7-positive compartments >=0.5 µm diameter. The same compartments marked in (A–C) are followed over time in (D–F). (G) Quantification of compartments as in (A–F) for all genotypes per 500 μm2 area. (H) Quantification of Venus-Rab7 compartments (individual distinguishable green punctae) per photoreceptor axon terminal in the brain for the indicated genotypes. (I) Quantification of the fraction of compartments that underwent green-to-red conversion over a 5 min period in same data as (G). Note that Rab7-T22N and Rab7-K157N did not mark sufficient compartments for this analysis. See also: Videos 1–10. Scale bar in (A): 10 µm.