Fig 3. Non-viable recombinants express representative immediate early and early, but not late genes.

Fibroblasts were transfected with the indicated recombinant genomes and a pp71-expression plasmid and thirteen days later, cells grown on coverslips were stained for the indicated viral antigen (red) and visualized by indirect immunofluorescence microscopy. Nuclei were stained with DAPI (blue) and are displayed in merged images to the left of each viral antigen. Representative images are shown.