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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Virology. 2013 Oct 24;0:10.1016/j.virol.2013.10.011. doi: 10.1016/j.virol.2013.10.011

Fig 4. Non-viable recombinants fail to replicate viral DNA.

Fig 4

Total DNA was extracted either one or thirteen days post transfection (dpt) of fibroblasts with the indicated recombinant BAC genomes. Aliquots were digested (+) or not (-) with DpnI. The MIEP region was amplified by PCR, products were separated by agarose gel electrophoresis and stained with ethidium bromide. M, size markers.