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Western blot of cell lines for thymidylate synthase (TS). Cytosolic and nuclear extracts were prepared from cell lines H23-Ct and H23-R1. They were separated in polyacrylamide gels, transferred to membranes, and probed with antibodies directed to TS, Oct-1 (a nuclear protein), and GAPDH (a cytosolic protein). A band of 38 kD representing TS was found in the cytoplasm.
