Figure 2.

Each miR-29 member inhibits cell proliferation and induces cell apoptosis and AKT2 and CCND2 are validated as targets of miR-29 family in the AML cell lines. (a) Cell grow curve of THP1 and NB4 transfected with miR-29a, -29b, -29c or control mimic. The results were shown as mean±S.D. of three independent experiments. (b) Annexin V/PI assays in THP1 and NB4 after transfection with the mimics for 72 h. The lower-left region represents the living cells, the lower-right region represents cells in early apoptosis and the upper-right region represents the late apoptotic cells or dead cells. (c) Statistical analysis of the percentage of Annexin V-positive cells in the cells transfected with miR-29a, -29b or -29c versus that in the cells transfected with control mimic (scr). Data are the average of three independent experiments±S.D. *P<0.05; **P<0.01; ***P<0.0001. (d) The nucleotide sequences of miR-29a, the predicted binding sites of miR-29 members and the mutated nucleotides (underlined) in the 3′UTR of AKT2 and CCND2 were shown. (e) Dual luciferase activity in the transfected 293T cells. The results were shown as mean±S.D. of three separated experiments. (f) Immunoblotting of Akt2 and CCND2 in THP1 and NB4 after transfection with miR-29s mimics or their inhibitors for 48 h