Figure 4.

MiR-29a/b/c expression was negatively correlated with the protein levels of target genes, AKT2 and CCND2, in the PBMNC samples. (a and b) Western blotting analysis of Akt2 and CCND2 expression in the PBMNC samples derived from 23 healthy donors and 20 AML patients. Quantification of the expression levels of Akt2 and CCND2 was performed by using the Image J software and normalized to β-actin. ***P<0.0001. (c) Taqmam stem-loop RT-RCR analysis of miR-29a, -29b and -29c expression in the PBMNC samples. ***P<0.0001. (d and e) Pearson correlation analysis between miR-29a/b/c and Akt2/CCND2 expression in the PBMNC samples. The Pearson correlation coefficient r was calculated and verified by the two-tailed significance test. ***P<0.0001