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. 2013 Sep 27;21(1):100–112. doi: 10.1038/cdd.2013.133

Figure 6.

Figure 6

Ectopic expression of miR-29s can partially correct abnormal cell proliferation and apoptosis inhibition in AML BM CD 34+ cells through targeting AKT2 and CCND2. (a) The relative expression of miR-29a, -29b, and -29c in BM CD34+ cells from five healthy donors and 20 primarily diagnosed AML patients were detected by Taqman stem-loop RT-PCR. U6 snRNA was used as the internal control. The PCR of each sample was performed in triplicate. The same sample was detected in each real-time PCR operation to eliminate the errors among different plates. ***P<0.0001. (b) Cell growth curves of BM CD34+ cells derived from three AML patients after infection with lenti_29a, lenti_29b, lenti_29c and lenti_con, respectively. (c) Annexin V/7-AAD assays of the BM CD 34+ cells after infection with the viruses for 4 days. (d) Western blotting analysis of Akt2 and CCND2 in the BM CD34+ cells after infection for 5 days