Figure 6.

EWS KO mice show the alteration of Drosha, miRNAs, and Col4a1 and CTGF. (a) Drosha mRNA levels were increased in EWS KO mice. The data represent the average±S.E.M. of three separate experiments. (b) miR-29b and miR-18b levels were markedly increased in EWS KO mice. (c) Col4a1 and CTGF mRNA levels were reduced in EWS KO mice. The data represent the average±S.E.M. of three separate experiments. Significantly different at *, P<0.05; **, P<0.005. (d) The protein levels of Drosha were increased in EWS KO mice whereas the protein levels of Col4a1 and CTGF were downregulated in the skin tissues of EWS KO mice (n=2). (e) Nissl staining showed severely altered skin structure of EWS KO mice. (f) Col4a1 and CTGF immunoreactivity were decreased in EWS KO mice skin tissues. All scale bars: 100 μm. (g) A schematic diagram illustrates that the upregulation of Drosha under EWS deficiency accelerates the processing of pri-miR-29b and pri-miR-18b in the nucleus. Increased miR-29b and miR-18b negatively regulate the mRNAs of Col4a1 and CTGF in the cytoplasm. As a result, EWS deficiency leads to impaired dermal development