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Schematic of fabrication strategy for layered collagen networks. (A) Collagen gels were neutralized in a rectangular mold (10 × 7 × 0.4 cm) in a gelation buffer, GB, at 4°C for 24 h. (B) Gels were incubated in fibril incubation buffer, FIB, at 37°C for 48 h. (C) 4mm thick gels were washed in DI water, and (D) dried to dense micron thick matrices. (E) Subsequent gels were dried atop dried matrices to generate multilayer constructs (F).
