Figure 1.

KLF8 immunolocalization in human placental villous tissues during early pregnancy. A total of 18 placentas, from 6 to 11 weeks, were used (3 different samples for each gestational age). A-C, Villous tissues at 6, 9, and 11 weeks immunostained (brown staining) with anti-KLF8 antibody as described in Materials and Methods. D-F, Negative control for each week treated with nonimmune rabbit Immunoglobulin G. Note that immunoreactive KLF8 was more intense at 9 weeks, compared with 6 and 11 weeks. All sections were counterstained with hematoxylin. Scale bar, 100 μm. Ct, cytotrophoblast; KLF8, Krüppel-like factor 8; St, syncytiotrophoblast.